ChAP Results

So my ChAP experiment didn’t work very well. I didn’t see the result I expected and I didn’t get a very high percent of purification. Previous ChAP experiments have gotten around 0.7-1.0 % of an input sample whereas I got around 0.1%. My results contained a high degree of standard deviation as well which shouldn’t be the case with qPCR. Normally, qPCR produces highly accurate and highly precise results. Since my results weren’t very precise, I’m hoping that I just messed up the qPCR part and not the entire experiment. To check this, I’m going to repeat the qPCR part since I still have left over sample. This should only take me a day or two so it’s not as time extensive as repeating the whole experiment again.