Finally Got Some Good Results

The double depletions did the trick. I got the results I expected with one of the siRNAs I was using. However, the new one I was using, the important one, didn't show the same results. I think I know why this is and I don't think it's a major problem so the results from this experiment are good news. Basically, my mistake was using too many cells. If I use too many cells, my siRNA will basically get diluted and not work as well as it should if I use less cells. So the only thing I have to do is use less cells next time and I should see the result I'm hoping for. Things are finally going in the right direction.